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only those wells that contain Human SP-D
And then biotinylated Human EAF2 Antibody is added and binds to EAF2 in the sample
GPIHBP1 present in the sample is added and binds to antibodies coated on the wells
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human EPO
Human SPON2 (Spondin-2) ELISA Kit Packaging:96 wells only those wells that containThe Human (SPON2) Spondin 2 ELISA Kit measures Spondin 2 in samples. The plate has been pre coated with Human Spon2 antibody. Spon2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human Spon2 Antibody is added and binds to Spon2 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated Spon2 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
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