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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PF4
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CTSD
Then Streptavidin-HRP is added and binds to the Biotinylated HAS3 antibody
FGFBP1 present in the sample is added and binds to antibodies coated on the wells
Human ICOSLG (Icos Ligand) ELISA Kit Packaging:96 wells Standards or samples are addedThe Human (ICOSLG) Icos Ligand ELISA Kit measures ICOS ligand in samples. The plate has been pre coated with Human ICOSLG antibody. ICOSLG present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human ICOSLG Antibody is added and binds to ICOSLG in the sample. Then Streptavidin HRP is added and binds to the Biotinylated ICOSLG antibody. After incubation unbound Streptavidin HRP is washed away during a washing
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