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The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse a1AT
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ROMO1
The concentration of Mouse LFA1a in the samples is then determined by comparing the OD of the samples to the standard curve
only those wells that contain Human IL1q
Human CHIA (Acidic Mammalian Chitinase) ELISA Kit UM The microtiter plate provided inThe Human (CHIA) Acidic Mammalian Chitinase ELISA Kit measures Acidic Mammalian Chitinase in samples. The plate has been pre coated with Human CHIA antibody. CHIA present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human CHIA Antibody is added and binds to CHIA in the sample. Then Streptavidin HRP is added and binds to the Biotinylated CHIA antibody. After incubation unbound Streptavidin HRP is washed away
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