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Then a biotinylated detection antibody specific for Rat NGAL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
then it binds with ACA-IGG bound to precoated antibody
The plate has been pre-coated with Bovine CHI3L1 antibody
Substrate solution is then added and color develops in proportion to the amount of Rat betaGD
Porcine PP (Pepsin) ELISA Kit D17S136E Then a biotinylated detection antibodyThe Porcine PP (Pepsin) ELISA Kit measures PP in Porcine Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti PP antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it
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