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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IL25
Only those wells that contain Human Flt3L
Then a biotinylated detection antibody specific for Human PDGF-AB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
When the sample containing C1s is added to the plate
Chicken MSTN (Myostatin) ELISA Kit weche Standards or samples are addedThe Chicken (MSTN) Myostatin ELISA Kit measures Myostatin in Chicken samples. The plate has been pre coated with Chicken MSTN antibody. MSTN present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Chicken MSTN Antibody is added and binds to MSTN in the sample. Then Streptavidin HRP is added and binds to the Biotinylated MSTN antibody. After incubation unbound Streptavidin HRP is washed away during a washing
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