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the TMB substrate (enzyme substrate) is added which reacts with HRP to form a blue-colored product that is inversely proportional to the amount of 3alpha-Diol G present
Then Streptavidin-HRP is added and binds to the Biotinylated CRKL antibody
The concentration of PLEC in the sample was calculated by drawing a standard curve
The microplate provided in this kit has been precoated with anti RGS6 antibody
Human ERK3 (Extracellular Signal Regulated Kinase 3) ELISA Kit C3a the TMB substrate (enzyme substrate)The Human ERK3 (Extracellular Signal Regulated Kinase 3) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Human ERK3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ERK3 and Avidin Horseradish Peroxidase (HRP) conjugate are added
