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CLDN15 present in the sample is added and binds to antibodies coated on the wells
The concentration of MCP2 in the samples is then determined by comparing the OD of the samples to the standard curve
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat FADS1
Then Streptavidin-HRP is added and binds to the Biotinylated ATP2B2 antibody
Mouse IL-33 (Interleukin 33) ELISA Kit Packaging:96 wells CLDN15 present in the sampleThe Mouse (IL 33) Interleukin 33 ELISA Kit measures Interleukin 33 in Mouse samples. The plate has been pre coated with Mouse IL33 antibody. IL33 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse IL33 Antibody is added and binds to IL33 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated IL33 antibody. After incubation unbound Streptavidin HRP is washed away during a washing
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