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Then a biotinylated detection antibody specific for Mouse SP-D and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
only those wells that contain Human APMAP
The concentration of Human FGF2 in the samples is then determined by comparing the OD of the samples to the standard curve
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL21R
Rat SDF-1Alpha (Stromal Cell Derived Factor 1Alpha) ELISA Kit Packaging:96 wells Then a biotinylated detection antibodyThe Rat SDF 1Alpha (Stromal Cell Derived Factor 1Alpha) ELISA Kit measures SDF 1Alpha in Rat Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti SDF 1 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add
