Human SLC25A37 (Mitoferrin-1) ELISA Kit HRGA Standards or samples are added

Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CD83

Substrate solution is then added and color develops in proportion to the amount of Bovine ANXA2

The concentration of Rat PRF1 in the samples is then determined by comparing the OD of the samples to the standard curve

And then biotinylated Human CPS1 Antibody is added and binds to CPS1 in the sample

Human SLC25A37 (Mitoferrin-1) ELISA Kit HRGA Standards or samples are addedThe Human (SLC25A37) Mitoferrin 1 ELISA Kit measures Mitoferrin 1 in samples. The plate has been pre coated with Human SLC25A37 antibody. SLC25A37 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human SLC25A37 Antibody is added and binds to SLC25A37 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated SLC25A37 antibody. After incubation unbound Streptavidin HRP is washed away