Human KLB (Klotho Beta) ELISA Kit Packaging:96 wells Only those wells that contain

Only those wells that contain Human COL1α2

Then a biotinylated detection antibody specific for HO1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

only those wells that contain Mouse SYN2

Substrate solution is then added and color develops in proportion to the amount of Bovine PF-4

Human KLB (Klotho Beta) ELISA Kit Packaging:96 wells Only those wells that containThe Human (KLB) Klotho Beta ELISA Kit measures Klotho Beta in samples. The plate has been pre coated with Human KLB antibody. KLB present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human KLB Antibody is added and binds to KLB in the sample. Then Streptavidin HRP is added and binds to the Biotinylated KLB antibody. After incubation unbound Streptavidin HRP is washed away during a washing step. Substrate