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Substrate solution is then added and color develops in proportion to the amount of Mouse FSTL3
Then a biotinylated detection antibody specific for Mouse CTSS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Then Streptavidin-HRP is added and binds to the Biotinylated C1orf35 antibody
RFTN1 present in the sample is added and binds to antibodies coated on the wells
Rat DHEA (Dehydroepiandrosterone) ELISA Kit Packaging:96 wells Substrate solution is then addedThe Rat DHEA (Dehydroepiandrosterone) ELISA Kit measures DHEA in Rat Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Competitive ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with DHEA. DHEA in the sample or standard competes with a fixed amount of biotin labeled DHEA for sites on a pre coated antibody specific to DHEA. Free components are washed off. After
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