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Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human LTF are added to the micro ELISA plate wells
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PF4
The concentration of Rat ADIPOR2 in the samples is then determined by comparing the OD of the samples to the standard curve
only those wells that contain Mouse IL1a
Human FZD2 (Frizzled-2) ELISA Kit Gamma-seminoprotein Samples (or Standards) and HorseradishThe Human (FZD2) Frizzled 2 ELISA Kit measures Frizzled 2 in samples. The plate has been pre coated with Human FZD2 antibody. FZD2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human FZD2 Antibody is added and binds to FZD2 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated FZD2 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
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