Substrate solution is then added and color develops in proportion to the amount of Human FAM3D
The concentration of CX3CL1 in the samples is then determined by comparing the OD of the samples to the standard curve
only those wells that contain Mouse RAB5A
Then a biotinylated detection antibody specific for Human DKK1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
EDN1 (Endothelin-1) ELISA Kit Packaging:96 wells Substrate solution is then addedThe EDN1 (Endothelin 1) ELISA Kit measures ET 1 in Mouse, Rat, Rabbit, Goat, Canine, Human, Porcine, Bovine Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti ET 1 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound