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a horseradish peroxidase conjugated mouse anti-Human IL-4 Antibody is added to the wells and binds to IL-4 in the sample
And then biotinylated Human BLNK Antibody is added and binds to BLNK in the sample
You can calculate the concentration of Human KIM-1 in the samples by comparing the OD of the samples to the standard curve
Then Streptavidin-HRP is added and binds to the Biotinylated HABP2 antibody
Rat GDF2 (Growth Differentiation Factor 2) ELISA Kit Packaging:96 wells a horseradish peroxidase conjugated mouseThe Rat GDF2 (Growth Differentiation Factor 2) ELISA Kit measures GDF2 in Rat Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti GDF2 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated
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