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Calculate the concentration of IGJ in the sample by plotting standard curve
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Zebrafish IFNg
You can calculate the concentration of Human TG in the samples by comparing the OD of the samples to the standard curve
it binds with GAL4 bound to precoated antibody
FFA (free fatty acid) ELISA Kit Packaging:96 wells Calculate the concentration of IGJThe FFA (free fatty acid) ELISA Kit measures FFA in Universal Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Competitive ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with FFA. FFA in the sample or standard competes with a fixed amount of biotin labeled FFA for sites on a pre coated antibody specific to FFA. Free components are washed off. After adding HRP
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