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Then a biotinylated detection antibody specific for Rat BDNF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
You can calculate the concentration of Rat ADP/Acrp30 in the samples by comparing the OD of the samples to the standard curve
ACO1 present in the sample is added and binds to antibodies coated on the wells
The concentration of Mouse GDF1 in the samples is then determined by comparing the OD of the samples to the standard curve
Rat KRT15 (Keratin 15) ELISA Kit Packaging:96 wells Then a biotinylated detection antibodyThe Rat (KRT15) Keratin 15 ELISA Kit measures Keratin 15 in Rat samples. The plate has been pre coated with Rat KRT15 antibody. KRT15 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat KRT15 Antibody is added and binds to KRT15 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated KRT15 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
