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Substrate solution is then added and color develops in proportion to the amount of Human AKR1C4
Calculate the concentration of IL-6Rα in the sample by plotting standard curve
And then biotinylated Chicken cath-K Antibody is added and binds to cath-K in the sample
a horseradish peroxidase conjugated mouse anti-Human SULT1E1 Antibody is added to the wells and binds to SULT1E1 in the sample
Rat STC1 (Stanniocalcin-1) ELISA Kit Packaging:96 wells Substrate solution is then addedThe Rat STC1 (Stanniocalcin 1) ELISA Kit measures STC1 in Rat Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti Stc1 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody.
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