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Calculate the concentration of ASL in the sample by plotting standard curve
The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ELN
The concentration of Rat IFNg in the samples is then determined by comparing the OD of the samples to the standard curve
Sheep HSP 70 (Heat Shock Protein 70) ELISA Kit EDF Calculate the concentration of ASLThe Sheep HSP 70 (Heat Shock Protein 70) ELISA Kit measures HSP 70 in Sheep Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti HSP70 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated
