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Human IgG4 in the sample or standard competes with a fixed amount of Human IgG4 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human IgG4
only those wells that contain Human F12
The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 14 nm
a horseradish peroxidase conjugated mouse anti-Human CD93 Antibody is added to the wells and binds to CD93 in the sample
ICAM4 (Intercellular Adhesion Molecule 4) ELISA Kit (Small Sample) Packaging:48 wells Human IgG4 in the sampleThe ICAM4 (Intercellular Adhesion Molecule 4) ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Human ICAM4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Human ICAM4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate
