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Substrate solution is then added and color develops in proportion to the amount of Rat IGFBP3
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human INa
Then Streptavidin-HRP is added and binds to the Biotinylated MAN1A1 antibody
And then biotinylated Mouse VCL Antibody is added and binds to VCL in the sample
Mouse ATG10 (Autophagy Related Protein 10) ELISA Kit Packaging:96 wells Substrate solution is then addedThe Mouse (ATG10) Autophagy Related Protein 10 ELISA Kit measures Autophagy related protein 10 in Mouse samples. The plate has been pre coated with Mouse ATG10 antibody. ATG10 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse ATG10 Antibody is added and binds to ATG10 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated ATG10 antibody. After incubation unbound Streptavidin
