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Mouse IgG2b in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Mouse IgG2b
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sheep IL12
BIRC3 present in the sample is added and binds to antibodies coated on the wells
only those wells that contain Human NF2
Human FEV (Protein Fev) ELISA Kit Packaging:96 wells Mouse IgG2b in samples (orThe Human (FEV) Protein Fev ELISA Kit measures Protein FEV in samples. The plate has been pre coated with Human FEV antibody. FEV present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human FEV Antibody is added and binds to FEV in the sample. Then Streptavidin HRP is added and binds to the Biotinylated FEV antibody. After incubation unbound Streptavidin HRP is washed away during a washing step. Substrate
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