Rat TPH2 (Tryptophan 5-hydroxylase 2) ELISA Kit Packaging:96 wells Substrate solution is then added

Substrate solution is then added and color develops in proportion to the amount of Bovine IL-7

The concentration of Human IL39 in the samples is then determined by comparing the OD of the samples to the standard curve

Then Streptavidin-HRP is added and binds to the Biotinylated HTRA1 antibody

And then biotinylated Mouse OLR1 Antibody is added and binds to OLR1 in the sample

Rat TPH2 (Tryptophan 5-hydroxylase 2) ELISA Kit Packaging:96 wells Substrate solution is then addedThe Rat (TPH2) Tryptophan 5 hydroxylase 2 ELISA Kit measures Tryptophan 5 hydroxylase 2 in Rat samples. The plate has been pre coated with Rat TPH2 antibody. TPH2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat TPH2 Antibody is added and binds to TPH2 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated TPH2 antibody. After incubation unbound Streptavidin HRP is washed away