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The concentration of Mouse PRG4 in the samples is then determined by comparing the OD of the samples to the standard curve
And then biotinylated Mouse SFRP5 Antibody is added and binds to SFRP5 in the sample
And then biotinylated Human JAG2 Antibody is added and binds to JAG2 in the sample
The concentration of Human TNFb in the samples is then determined by comparing the OD of the samples to the standard curve
Human CALD (Caldesmon) ELISA Kit Packaging:48 wells The concentration of Mouse PRG4The Human CALD (Caldesmon) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Human CALD. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CALD and Avidin Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and
