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and the color intensity is directly proportional to the amount of Human CTHRC1 present
a solution of TMB Reagent is added and incubated at room temperature for 20 minutes
Then a biotinylated detection antibody specific for Human PPAR-Gamma and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The Rabbit CCL4/MIP1b ELISA kit is a sandwich enzyme immunoassay
Mouse IL1b (Interleukin 1 Beta) ELISA Kit (Small Sample) Packaging:96 wells and the color intensity isThe Mouse IL1b (Interleukin 1 Beta) ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Mouse IL1b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Mouse IL1b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is
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