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The concentration of Human PGI in the samples is then determined by comparing the OD of the samples to the standard curve
The absorbance values are measured at 450 nm (optionally 450/630 nm) and are proportional to the concentration of Apo M in the specimen
coli expression system and the target gene encoding Ile22-Ala212 is expressed
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse S100
Human CNTN5 (Contactin-5) ELISA Kit Packaging:96 wells The concentration of Human PGIThe Human (CNTN5) Contactin 5 ELISA Kit measures Contactin 5 in samples. The plate has been pre coated with Human CNTN5 antibody. CNTN5 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human CNTN5 Antibody is added and binds to CNTN5 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated CNTN5 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
