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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to TNFSF12
Then a biotinylated detection antibody specific for Canine TNF-Alpha and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Then Streptavidin-HRP is added and binds to the Biotinylated TAC4 antibody
Only those wells that contain Porcine NUP160
Rat PRKAA1 (Protein Kinase, AMP Activated Alpha 1) ELISA Kit Packaging:96 wells Standards or samples are addedThe Rat PRKAA1 (Protein Kinase, AMP Activated Alpha 1) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Rat PRKAA1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PRKAA1 and Avidin Horseradish Peroxidase (HRP) conjugate are added successively
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