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Then a biotinylated detection antibody specific for Rat NRP1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The concentration of Human sICAM1 in the samples is then determined by comparing the OD of the samples to the standard curve
The concentration of Rat VAP-1 in the samples is then determined by comparing the OD of the samples to the standard curve
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KCNN2
Human APC (Adenomatosis Polyposis Coli Protein) ELISA Kit Packaging:96 wells Then a biotinylated detection antibodyThe Human (APC) Adenomatosis Polyposis Coli Protein ELISA Kit measures Adenomatosis Polyposis Coli Protein in samples. The plate has been pre coated with Human APC antibody. APC present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human APC Antibody is added and binds to APC in the sample. Then Streptavidin HRP is added and binds to the Biotinylated APC antibody. After incubation unbound Streptavidin HRP is
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