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Mouse VIP in the sample or standard competes with a fixed amount of Mouse VIP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse VIP
Calculate the concentration of CD14 in the sample by plotting standard curve
Then Streptavidin-HRP is added and binds to the Biotinylated RORC antibody
Calculate the concentration of H3-4 in the sample by plotting standard curve
Mouse UCP-2 (Uncoupling Protein 2, Mitochondrial) ELISA Kit Apoptain Mouse VIP in the sampleThe Mouse UCP 2 (Uncoupling Protein 2, Mitochondrial) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Mouse UCP 2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse UCP 2 and Avidin Horseradish Peroxidase (HRP) conjugate are added successively
