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Calculate the concentration of Cyb5r3 in the sample by plotting standard curve
The plate has been pre-coated with Mouse LEPTIN antibody
The absorbance values are measured at 450 nm (optionally 450/630 nm) and are proportional to the concentration of mCRP in the specimen
Then a biotinylated detection antibody specific for Mouse Hepc and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Human HO1 (Heme Oxygenase 1) ELISA Kit IL27p28 Calculate the concentration of Cyb5r3The Human HO1 (Heme Oxygenase 1) ELISA Kit measures HO1 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti HO1 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody.
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