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Then a biotinylated detection antibody specific for Human F7 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
and the color intensity is directly proportional to the amount of Human GM-CSF present
The concentration of Human HNF1a in the samples is then determined by comparing the OD of the samples to the standard curve
The Human (CHKB) Choline Ethanolamine Kinase ELISA Kit measures Choline/ethanolamine kinase in samples
Human CNFN (Cornifelin) ELISA Kit Packaging:96 wells Then a biotinylated detection antibodyThe Human (CNFN) Cornifelin ELISA Kit measures Cornifelin in samples. The plate has been pre coated with Human CNFN antibody. CNFN present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human CNFN Antibody is added and binds to CNFN in the sample. Then Streptavidin HRP is added and binds to the Biotinylated CNFN antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
