Human IK (Protein Red) ELISA Kit MRP14 Substrate solution is then added

Substrate solution is then added and color develops in proportion to the amount of Mouse CLDN4

The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-1Beta

and the Rat VEGFR2 standard plate wells that pre-coated using protein-related techniques are provided separately

and the color intensity is directly proportional to the amount of Human CXCL9 present

Human IK (Protein Red) ELISA Kit MRP14 Substrate solution is then addedThe Human (IK) Protein Red ELISA Kit measures Protein Red in samples. The plate has been pre coated with Human IK antibody. IK present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human IK Antibody is added and binds to IK in the sample. Then Streptavidin HRP is added and binds to the Biotinylated IK antibody. After incubation unbound Streptavidin HRP is washed away during a washing step. Substrate solution