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And then biotinylated Mouse DMD Antibody is added and binds to DMD in the sample
Then a biotinylated detection antibody specific for Human HMGB-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Then Streptavidin-HRP is added and binds to the Biotinylated AURKA antibody
Substrate solution is then added and color develops in proportion to the amount of Rat ACTB
Human BFP (Brain Finger Protein) ELISA Kit Packaging:48 wells And then biotinylated Mouse DMDThe Human BFP (Brain Finger Protein) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Human BFP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human BFP and Avidin Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate
