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Then a biotinylated detection antibody specific for Mouse S100A8 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Substrate solution is then added and color develops in proportion to the amount of Sheep AVP
The assay plate has been pre-coated with mouse anti-Human Angiotensinogen monoclonal antibody
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL26
Human STX5 (Syntaxin 5) ELISA Kit Packaging:96 wells Then a biotinylated detection antibodyThe Human (STX5) Syntaxin 5 ELISA Kit measures Syntaxin 5 in samples. The plate has been pre coated with Human STX5 antibody. STX5 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human STX5 Antibody is added and binds to STX5 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated STX5 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
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