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The substrate solution is added to each well
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SFRP1
Then Streptavidin-HRP is added and binds to the Biotinylated NPPB antibody
Only those wells that contain Guinea pig CD109
Rabbit PKP1 (Plakophilin 1) ELISA Kit Scurfin The substrate solution is addedThe Rabbit PKP1 (Plakophilin 1) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Rabbit PKP1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rabbit PKP1 and Avidin Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate
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