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Shake / stir for 12-24 hours in 4- 5% PPM™ solution supplemented with full strength basal salts without pH ing & Tween 20
Callus-mediated shoot regeneration
it serves a few different purposes:
and deficiency responses
Murashige & Skoog (MS) Basal Salts - Modification 2 Size:10 units of 1 LT Shake / stir for 12-24Murashige & Skoog (MS) Basal Salts Modification 2 Murashige & Skoog medium supplemented with vitamins but not containing glycine offers a refined nutrient environment that supports the growth and development of various plant tissues in culture. The addition of vitamins, such as thiamine, nicotinic acid, and pyridoxine, enhances cellular metabolism and promotes overall plant vitality, making this medium particularly effective for tissue culture
